Author/Editor		Grabner, Sabina; Košmrlj, Janez; Bukovec, Nataša; Čemažar, Maja
Title		Pt(II) complexes with N-(3-pyridyl)-2-(4-(trifluoromethyl)phenyl)diazenecarboxamide and their reactions with glutathione
Type		članek
Source		J Inorg Biochem
Vol. and No.		Letnik 95
Publication year		2003
Volume		str. 105-12
Language		eng
Abstract		The reaction between [PtCI(dmso)(en)]Cl (dmso=dimethyl sulfoxide, en=ethylenediamine) and N-(3-pyridyl)-2-(4-(trifluoromethyl)phenyl)diazenecarboxamide (L) was studied using multinuclear NMR spectroscopy. The water-soluble complexes (PtCI(en)(LNI )]+ (1) and [Pt(en)(L-NI)2]2+ (2) were isolated and their reactions with glutathione (GSH) were investigated to assess the oxidation properties of coordinated L. Both species 1 and 2 oxidized GSH to GSSG, while the reduced form of L (semicarbazide, SL) remained coordinated to Ptz' . In complex 1 the labile chloride ion was substituted by the thiol moiety of GSH, which gave rise to the release of en in excess GSH over a period of 7 days. Complexes [PtCI(dmso)(en)]Cl, 1, 2 and ligand L were tested against T24 bladder carcinoma cells. Ligand L and complexes 1 and 2 showed higher cytotoxicity than [PtCI(dmso)(en)]CI.
Descriptors		GLUTATHIONE IMIDES ORGANOPLATINUM COMPOUNDS PLATINUM PYRIDONES ANTINEOPLASTIC AGENTS BLADDER NEOPLASMS DOSE-RESPONSE RELATIONSHIP, DRUG HYDROCARBONS, CHLORINATED MAGNETIC RESONANCE IMAGING MOLECULAR STRUCTURE OXIDATION-REDUCTION SULFHYDRYL COMPOUNDS TUMOR CELLS, CULTURED TIME FACTORS