Author/Editor		Jarm, Tomaž; Serša, Gregor; Miklavčič, Damijan
Title		Oxygenation and blood flow in tumors treated with hydralazine: evaluation with a novel luminescence-based fiber-optic sensor
Type		članek
Source		Technol Health Care
Vol. and No.		Letnik 10
Publication year		2002
Volume		str. 363-80
Language		eng
Abstract		Two optical methods were used simultaneously to evaluate the effect of a vasoactive drug hydralazine on oxygenation and blood flow in SA-1 tumors in A/J mice. A novel luminescence-based optical sensor (OxyLite instrument, Oxford Optronix, UK) was used to monitor partial pressure of oxygen (pO2) in tumors. Laser Doppler flowmetry was used to assess relative blood perfusion in tumors. Measurements were performed continuously on anesthetized mice before and after i.v. injection of hydralazine at a dose of 2.5 mg/kg. Hydralazine reduced p` on average by 80% and blood flow by 50% within ten minutes after injection. Our results indicate that hydralazine could be used to improve the antitumor effectiveness of hypoxic cell-specific therapies. We demonstrated that both optical methods can be used successfully to detect changes in blood perfusion and oxygenation in tumors after blood flow-modifying treatments. Some aspects of pO2 measurement with the new luminescence-based method require further investigation.
Descriptors		FIBROSARCOMA HYDRALAZINE NEOPLASMS, EXPERIMENTAL OXYGEN CONSUMPTION VASODILATOR AGENTS MICE INJECTIONS, INTRAVENOUS LASER-DOPPLER FLOWMETRY REGIONAL BLOOD FLOW