Author/Editor		Berovič, Marin; Habijanič, Jožica; Zore, Irena; Wraber, Branka; Hodžar, Damjan; Boh, Bojana; Pohleven, Franc
Title		Submerged cultivation of Ganoderma lucidum biomass and immunostimulatory effects of fungal polysaccharides
Type		članek
Source		J Biotechnol
Vol. and No.		Letnik 103
Publication year		2003
Volume		str. 77-86
Language		eng
Abstract		Original Ganoderma lucidum strain MZKI G97 isolated from Slovenian forests was cultivated in a liquid substrate based on potato dextrose and olive oil. The influences of inoculum and oxygen partial pressure in batch and fed batch cultivation in a 10-1 laboratory stirred tank reactor were studied. Fungal biomass was found to be oxygen and shear sensible. Using a 17%, (wet weight) 6 days old vegetative inoculum, 9.6 g 1-1 of dry biomass in batch cultivation and 15.2 g 1-1 in fed batch process were obtained. Extracellular (9.6 g 1-1) and intracellular (6.3 g 1-1) polysaccharide fractions were isolated. Extracellular polysaccharide fraction and four intracellular polysaccharide fractions were obtained. Polysaccharides were further separated by ion-exchange, gel and affinity chromatography. The isolated polysaccharides were mainly beta-D-glucanes. Immunostimulatory effects of isolates were tested on induction of cytokine (tumour necrosis factor a (TNF-alpha) and interferon gamma (IFN-gamma)) synthesis in primary cultures of human peripheral blood mononuclear cells (PBMC) isolated from a buffy coat. The TNF-a inducing activity is comparable with romurtide, which has been used as a supporting therapy in cancer patients treated with radiotherapy and/or chemotherapy.
Descriptors		BASIDIOMYCETES BIOMASS POLYSACCHARIDES MONOCYTES CULTURE MEDIA IMMERSION CHROMATOGRAPHY TUMOR NECROSIS FACTOR INTERFERON TYPE II