Author/Editor		Dolenc-Koce, Jasna; Vilhar, Barbara; Bohanec, Borut; Dermastia, Marina
Title		Genome size of Adriatic seagrasses
Type		članek
Source		Aquat Bot
Vol. and No.		Letnik 77, št. 1
Publication year		2003
Volume		str. 17-25
Language		eng
Abstract		Genome size (C-value) was measured in four species of Adriatic seagrasses with interphase-peak DNA image cytometry. The estimated 2C-value was 1.5 pg DNA for Zostera noltii (2n = 12), 1.2 pg for Zostera marina (2n = 12), I .1 pg for Cymodocea nodosa (2n = 28) and 6.2 pg for Posidonia oceanica, using Pisum sativum (2C-value = 8.84 pg) as the calibration standard. Seagrass leaves were fixed in 4% buffered formaldehyde to mitigate stoichiometric error due to tannins and post-fixed in 3:1 methanol:acetic acid (MAA). DNA was stained with the Feulgen reaction after hydrolysis in 5 M HCl for 90 min at 20 degreesC. Comparison of genome size of seagrasses with the data for other species of Alismatidae indicated that the ancestral genome of Alismatidae was relatively small.
Descriptors		SEAWATER GRASSES GENOME, PLANT DNA IMAGE CYTOMETRY FLOW CYTOMETRY PHYLOGENY