| Author/Editor | Battelino, T; Uršič-Bratina, N; Dolžan, V; Stopar-Obreza, M; Pozzilli, P; Kržišnik, C; Vidan-Jeras, B | |
| Title | The HLA-DRB, -DQB polymorphism and anti-insulin antibody response in Slovenian patients with type 1 diabetes | |
| Type | članek | |
| Source | Eur J Immunogenet | |
| Vol. and No. | Letnik 30 | |
| Publication year | 2003 | |
| Volume | str. 223-7 | |
| Language | eng | |
| Abstract | A combination of specific HLA class II antigens and the presence of type 1 diabetes (TiD)-related antibodies has a high positive predictive value for T1D but low sensitivity. The aim of the present study was to determine the frequencies of HLA-DRB-DQB deduced haplotypes associated with susceptibility and protection in Slovenian patients with established T1D, to evaluate the relationship between the HLA-DRBI-QBP-DQBl haplotypes and the presence of insulin autoantihodies (IAA) and glutamic acid decarboxylase antibodies (GADA), and to access the possible impact of polymorphic QBP promoters on this relationship. A cohort of 135 patients with T1D (age 17.5 +- 7.0 years, duration of T1D 9.14 +- 6.3 years) was investigated. HLA-DRB1 and DQBl alleles were typed using the polymerase chain reaction (PCR)-reverse line blot method. QBP promoter region alleles were determined using PCR-sequence-specific oligonucleotide hybridization (SSO) and PCR-sequence-specific primers (SSP). IAA and GADA antibodies were determined by enzyme-linked immunosorbent assay (ELISA). The chisquare test with Yates' correction was used for statistical analysis. Deduced haplotypes DRBl*0301-DQBl*0201 (P -_ 0.0001, OR -_ 3.4), DRB1*0401-DQBl*0302 (P = 0.0001, OR . 29.8), and DRB1*0402-DQB1*0302 (P = 0.008, OR = 4.7) were significantly more common, and DRBl*1501-DQBl*0602 (P = 0.0001, OR = 0.03) significantly less common in the investigated cohort than in a Slovenian control group. The highest risk and the strongest protective HLA-DR-DQ haplotypes found in Slovenian patients with T1D did not differ from those found in other Caucasian populations. (Abstract truncated at 2000 characters) | |
| Descriptors | DIABETES MELLITUS, INSULIN-DEPENDENT HLA-DR ANTIGENS HLA-DQ ANTIGENS AUTOANTIBODIES INSULIN ANTIBODIES ANTIGENIC MODULATION POLYMERASE CHAIN REACTION ENZYME-LINKED IMMUNOSORBENT ASSAY HAPLOTYPES POLYMORPHISM (GENETICS) SLOVENIA |