Author/Editor		Petrovič, Nataša; Meng, B; Ravnikar, Maja; Mavrič, Irena; Korošec-Koruza, Zora; Tomažič, Irma; Gonsalves, D
Title		Prva detekcija virusnih delcev Rupestris stem pitting associated virus 1 (RSPAV-1) povezanih z boleznijo razbrazdanja lesa vinske trte
Translated title		First detection of Rupestris stem pitting associated virus particles (RSPAV-1) associated with the rugose wood disease of grapevine
Type		članek
Source		In: Maček J, editor. Zbornik predavanj in referatov 6. slovenskega posvetovanja o varstvu rastlin; 2003 mar 4-6; Zreče. Ljubljana: Društvo za varstvo rastlin Slovenije,
Publication year		2003
Volume		str. 289-9
Language		slo
Abstract		Rupestris stem pitting associated virus (RSPaV), a member of the Foveavirus genus, is associated with the Rupestris stem pitting component of the rugose wood (RW) disease complex of grapevines. Heretofore, particles of RSPaV have not been visualized. In this work, flexuous rod particles about 750 nm in length were detected in sap of infected grapevines, by immunosorbent electron microscopy (ISEM). Since particles were unstable in plant sap, selection of young shoot tissue and use of short incubation times were essential for observing intact particles. RSPaV particles were detected in tissue culture, greenhouse and field grown infected plants but not in healthy control plants. ISEM detection of particles corresponded to the presence of dsRNA, and detection of RSPaV by Western blot and ELISA. In contrast, the particles were not decorated by antibodies to GVA and GVB, two other viruses associated with RW. This first definitive detection of RSPaV particles will help towards understanding the etiology of the RW disease complex.
Summary		Rupestris stem pitting associated virus (RSPaV) spada v rod Foveavirus. Virus RSPaV-1 je povezan s tisto komponento bolezni razbrazdanja lesa vinske trte (Rugose wood disease complex, RW), ki povzroča razbrazdanje lesa na lesnem indikatorju Rupestris du Lot (Rupestris stem pitting). Do sedaj še nikomur ni uspelo dejansko tudi videti virusnih delcev RSPaV-1, kar bi predstavljalo prvi neposreden dokaz, da je dejavnik komponente razbrazdanja lesa resnično virusnega izvora. V soku z RSPaV-1 okuženih rastlin vinske trte smo s transmisijsko elektronsko mikroskopijo z metodo lovljenja (ISEM) in dekoracije s protitelesi proti rekombinantnemu virusnemu plaščnemu proteinu opazovali filamentozne virusne delce povprečne dolžine 750 nm. Virusne delce RSPaV smo zasledili v okuženih rastlinah vinske trte gojenih v tkivni kulturi, v rastlinjaku ter v vinogradu, nismo pa jih zasledili v kontrolnih zdravih rastlinah gojenih v istih razmerah. Rezultati ISEM se ujemajo z rezultati zastopanosti virusne dsRNA, metode imunskega pivnika (Western blot) in ELISA testa. Virusnih delcev RSPaV-1 z metodo ISEM nismo uspeli dekorirati s protitelesi proti virusoma Grapevine virus A (GVA) in Grapevine virus B (GVB), ki sta oba prav tako povezana z boleznijo razbrazdanja lesa vinske trte. Plod tega raziskovalnega dela predstavlja prvi neposreden dokaz o obstoju virusnih delcev RSPaV-1 in obeta pomembno orodje pri razumevanju etiologije te bolezni.
Descriptors		PLANT VIRUSES FRUIT POLYMERASE CHAIN REACTION ENZYME-LINKED IMMUNOSORBENT ASSAY MICROSCOPY, ELECTRON