Author/Editor		Vrtačnik-Bokal, Eda; Možina, Andrej; Poljak, Mario
Title		Humani virusi papiloma pri abnormnih ploščatih in blago diskariotičnih celicah
Translated title		Human papilloma virus in the abnormal squamous cells and mildy dyskaryotic cells
Type		članek
Source		Zdrav Vestn
Vol. and No.		Letnik 72, št. Suppl 2
Publication year		2003
Volume		str. II-55-9
Language		slo
Abstract		Background. A persistent infection with high-risk genotypes of human papilloma viruses (HPV) represents the most important etiologic factor for the development of cervical cancer, the second most frequent cancer in women in Slovenia as well as elsewhere in the world. in the detection of precancerous lesions the cervical Papanicolaou (Pap) smear screening is used in Slovenia and worldwide. Management of patients with repeat abnormal smears (Pap II) represents a great and complex clinical and public health problem; repeat cytologic examinations are the routine procedure in many countries, also in Slovenia, although the sensitivity of Pap smear testing in the detection of cervical intraepithelial neoplasms (CIN) II and III is relatively low. In cases of abnormal squamous cells and midlly dyskaryotic cells the presence of infections with high-risk HPV genotypes is being incresingly used as a complementary method to Pap smear testing. Methods and patients. In the study we enrolled 148 women who within two years had three subsequent Pap II smears (abnormal squamous cells or mildly dyskaryotic cells). The prevalence of HPV infections was determined using three molecular tests: Hybrid Capture II (HCII) (Digene Corporation, Gaithersburg, USA) and two variants of polymerase chain reaction (PCR-PGMY11/PGMY09 and PCR-CPI/CPIIG). HPV genotypes were determined using the method of enzyme restriction of PCR products multiplied by group-specific oligonucleotid primers PGMY11/PGMY09. Results. HPV infection was detected in 25.7% of women. In women aged <-30 years a statistically significantly higher incidence of HPV Infections was found (37.8%) than in women aged >-30 years (20.4%). Abstract truncated at 2000 characters.
Summary		Izhodišča. Dolgotrajna okužba z genotipi humanih virusov papiloma (HPV) z visokim tveganjem predstavlja najpomembnejši etiološki dejavnik za razvoj raka materničnega vratu (RMV), drugega najpogostejšega raka pri ženskah v Sloveniji. Za odkrivanje predrakavih sprememb uporabljamo v Sloveniji in v večini razvitih držav presejalni citološki test po Papanicolaouu (Pap). Obravnavanje bolnic s ponavljajočimi se rezultati testa Pap II predstavlja velik in kompleksen klinični in javni zdravstveni problem. V mnogih državah, tudi v Sloveniji, je ponavljanje citoloških preiskav v takih primerih ustavljena klinična praksa, čeprav je občutljivost citološkega testiranja za odkrivanje cervikalnih intraepitelijskih neoplazij stopenj II in III (CIN II, III) sorazmerno nizka. V svetu se kot dopolnilna metoda k citološkemu testiranju pri abnormnih ploščatih celicah in blagih diskariotičnih celicah vse pogosteje uporablja testiranje na prisotnost okužb z genotipi HPV z visokim tveganjem. Metode in preiskovanke. V našo raziskavo smo vključiči 148 žensk s tremi zaporednimi rezultati Pap II (abnormne ploščate celice ali blago diskariotične celice) presejalnega testa Pap v dveh letih. Prevalenco okužb s HPV smo ugotavljali s tremi molekularnimi testi: s testom tekočinske hibridizacije Hybrid Capture II (HCII) (Digene Corporation, Gaithersburg, ZDA) in z dvema različicama verižne reakcije s polimerazo (PCR-PGMY11/PGMY09 in PCR-CPI/CPIIG). Genotipe HPV smo opredelili z metodo encimske razgradnje pridelkov PCR, pomnoženih s skupinsko značilnimi oligonukleotidnimi začetniki PGMY11/PGMY09. Rezultati. Pri 25,7% žensk smo ugotovili okužbo s HPV. (Izvleček prekinjen pri 2000 znakih).
Descriptors		PAPILLOMAVIRUS, HUMAN SEXUALLY TRANSMITTED DISEASES, VIRAL VAGINAL SMEARS PREVALENCE POLYMERASE CHAIN REACTION CERVIX NEOPLASMS CARCINOMA, SQUAMOUS CELL GENOTYPE SLOVENIA